Subunit-specific polyclonal antibody targeting human ρ1 GABAC receptor
نویسندگان
چکیده
منابع مشابه
GABAC receptor-mediated inhibition is altered, but not eliminated in the superior colliculus of GABAC ρ1 knockout mice
GABAC receptors (GABACRs) are widely expressed in the mammalian subcortical visual system, particularly in the retina and superior colliculus (SC). GABACRs are composed of specific ρ1-3 subunits whose expression varies among visual structures. Thus, ρ1 subunits are most abundant in retina and their loss eliminates GABACR expression and function (McCall et al. J Neurosci 22:4163-4174). In the SC...
متن کاملMixed antagonistic effects of the ginkgolides at recombinant human ρ1 GABAC receptors
The diterpene lactones of Ginkgo biloba, ginkgolides A, B and C are antagonists at a range of Cys-loop receptors. This study examined the effects of the ginkgolides at recombinant human ρ(1) GABA(C) receptors expressed in Xenopus oocytes using two-electrode voltage clamp. The ginkgolides were moderately potent antagonists with IC(50)s in the μM range. At 10 μM, 30 μM and 100 μM, the ginkgolides...
متن کاملMonoclonal and Polyclonal Antibodies Specific to Human Fibromodulin
Background: The unique expression of fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL) has been previously reported. Detecting FMOD in CLL patients using specific anti-FMOD mAbs might provide a promising method in detection, monitoring, and prognosis of CLL. Objectives: In this study, we aimed for producing specific antibodies agains...
متن کاملCharacterization of a specific polyclonal antibody
Lipid hydroperoxide may react with protein or amino phospholipid without secondary decomposition. We prepared a polyclonal an tibody to lipid hydroperoxide-modified proteins using 13S-hydroperoxy-9Z, 1 1E-octadecadienoic acid-modified keyhole limpet hemocyanin (13-HPODEKLH) as immunogen. The antibody recognized 13-HPODEmodified bovine serum albumin (BSA), but not aldehydemodified proteins, such...
متن کاملDesign, Synthesis, and Pharmacological Evaluation of Fluorescent and Biotinylated Antagonists of ρ1 GABAC Receptors.
The ρ1 GABAC receptor is a ligand-gated chloride ion channel that shows promise as a therapeutic target for myopia, sleep disorders, memory and learning facilitation, and anxiety-related disorders. As such, there is a need for molecular probes to understand the role GABAC receptors play in physiological and pathological processes. To date, no labeled (either radioactive or fluorescent) GABAC se...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Experimental Eye Research
سال: 2011
ISSN: 0014-4835
DOI: 10.1016/j.exer.2011.04.009